housekeeping protein gapdh  (Bio-Rad)

Journal: Biology

Article Title: Ex Vivo Characterization of Peritoneal Macrophages from Novel ABCA1-LSL and ABCG1-LSL Mice for Macrophage-Specific ABC-Transporter Overexpression

doi: 10.3390/biology14081073

Figure Lengend Snippet: ABCG1-dependent cholesterol efflux is triggered in ABCG1-LSL +/0 PMs exposed to gesicles containing Cre: ( A ) Representative immunoblot of ABCG1 and GAPDH housekeeping protein for ABCG1-LSL +/0 PMs incubated with gesicles versus vehicle. ( B ) ABCG1 protein quantification from immunoblots. ( C ) Cholesterol efflux assessed in ABCG1-LSL +/0 PMs exposed to gesicles or vehicle only and then incubated with HDL. ( B , C ) Data points indicate biological replicates and originate from PMs isolated from 3 female ABCG1-LSL +/0 mice and 3 male ABCG1-LSL +/0 mice, with PMs plated equally and then subjected to treatment with vehicle only or gesicles. The uncropped western blot figures were presented in .

Article Snippet: Loading controls for our immunoblots included the housekeeping proteins GAPDH (1:750 dilution, sc-365062; Santa Cruz Biotechnology) and HSP90 (1:2500 dilution, 610419; BD Biosciences, San Jose, CA, USA).

Techniques: Western Blot, Incubation, Isolation

Journal: Molecular psychiatry

Article Title: The functional role of locus coeruleus microglia in the female stress response.

doi: 10.1038/s41380-025-02971-9

Figure Lengend Snippet: Fig. 3 Chemogenetic mediated suppression of microglial reactivity prevented the behavioral and neuroimmune responses to acute WS. A CNO treatment in rats administered the inhibitory microglial DREADDs virus in the LC reduced the burying response typically observed in response to repeated witness stress (WS). B CNO engagement of the inhibitory DREADD during stress prevented the WS-induced accumulation of the proinflammatory cytokine IL-1β in the LC. C Representative Western Blot lanes displaying IL-1β labeling (green) and the housekeeping protein to which it was normalized (GapDH, red). D In surgically naïve rats with no viral administration, CNO treatment alone did not lead to any alterations in burying behavior in WS exposed females. E Brain atlas location reflecting viral localization and expression which was restricted to the LC. *p < 0.05, **p < 0.008, ***p < 0.0002, ****p < 0.0001.

Article Snippet: Western Blots were probed for the antibodies IL-1β (1:200, goat, R & D Systems #AF-501-NA) and the housekeeping protein GapDH (1:1000, mouse, Santa Cruz #sc-365062) overnight followed by secondary antibodies (IR-Dye anti-rabbit 800 nm (LI-Cor #926–32213) and IR-Dye anti-mouse 680 nm (LI-Cor #962–68072) diluted 1:20,000) for 1 h. Membranes were imaged on a LI-Cor Odyssey scanner (LI-Cor Biotechnology, Lincoln, NE), and protein expression was quantified using LI-Cor Image Studios Software with normalization to GapDH.

Techniques: Virus, Western Blot, Labeling, Expressing